In this article we sum up a list of reagents, instruments and consumables that can be implemented in the detection of coronavirus. In order to better detect the new coronavirus, MOLBASE has specially cooperated with several major brand suppliers to jointly launch the following series of consumables, reagents and instruments to help you quickly and accurately diagnose the new coronavirus.

Please feel free to contact international@molbase.com for any inquiry or order place.

Three steps to detect coronavirus

No.1 Specimen Collection

No.2 nucleic acid extraction

No.3 Real-time RT-PCR

Each step of the experiment may lead to false negative test results, for example, it may be caused by the following reasons:

1. In the early stage of the disease, the viral load (quantity) is too small. Or the amount of virus in the collected specimen is too small;

2. RNA viruses are easily degraded when exposed to the air. Due to improper operation when collecting specimens, or the experimental consumables have not been treated with RNase-free, the specimens are degraded before detection;

3. The PCR amplification efficiency is too low and the CT value of the RT-PCR result is too high due to insufficient technical level of the experimenter or improper selection of reagents.

In order to better detect the new coronavirus, MOLBASE has specially cooperated with several major brand suppliers to jointly launch the following series of consumables, reagents and instruments to help you quickly and accurately diagnose the new coronavirus.

Laboratory Consumables (PCR)

Centrifuge tube (Corning)

Octal tube (Corning)

96-well plate (Corning)

Sealing film (Corning)

Conventional tip

Filter tip

Reagents and instruments (nucleic acid extraction)

The automatic extraction program

1. S32 Magnetic Bead Method Virus DNARNA Extraction Kit (DP604)

With the S32 automatic nucleic acid extraction instrument, it can isolate and purify high-quality virus DNA / RNA from serum, plasma, lymph fluid, acellular body fluid, cell culture supernatant, urine or various virus preservation solutions.

2. S96 Magnetic Bead Method Virus DNA_RNA Extraction Kit (DP804)

It is equipped with S96 automatic nucleic acid extraction and purification instrument to isolate and purify high-quality viral DNA / RNA from serum, plasma, lymph fluid, acellular body fluid, cell culture supernatant, urine or various virus preservation solutions.

3. Viral genomic DNA extraction kit (OSR-M202, M202-T1)

The M16 / M48 automatic nucleic acid extraction instrument is designed to isolate and purify high-quality viral DNA / RNA from serum, plasma, lymphatic fluid, acellular body fluid, cell culture supernatant, urine or various virus preservation solutions.

4. Tissue grinding homogenizer (OSE-TH-01)

For grinding and homogenizing before extraction of various samples, efficient processing of 1-24 independent grinding tube samples simultaneously

5. S32 automatic nucleic acid extraction instrument (YOSE-S32)

For automated extraction of viral DNA / RNA, 32 samples can be extracted simultaneously.

6. S96 Automatic Nucleic Acid Extraction and Purification Instrument (OSE-S96-01 / 02/03)

For automated extraction of viral DNA / RNA, 96 samples can be extracted simultaneously

7. M16 / M48 Automatic Nucleic Acid Extraction and Purification Instrument (OSE-M16 / M48)

For automated extraction of viral DNA / RNA, can extract 16/48 samples simultaneously

The manual extraction scheme

1. Viral Genomic DNA / RNA Extraction Kit (DP315)

Isolate and purify virus DNA or RNA from samples such as plasma, serum, and other cell-free body fluids

2. Viral RNA Extraction Kit (DP315-R)

Rapid extraction of viral RNA from plasma / serum / lymph

3. RNAStore sample preservation solution (DP408)

For preservation of tissue samples, tissues can be quickly immersed in RNAstore for storage without causing degradation of RNA

4. Electric tissue grinder set (OSE-Y30 / Y40 / Y50)

Hand-held grinder for rapid grinding and homogenization before sample extraction

5. Heating / cooling type five-stage programmable metal bath (OSE-DB-01 / 02/03)

Fast temperature rise and drop, precise temperature control, used for sample pyrolysis and other insulation operations during extraction

6. Blue light transmission meter / electrophoresis monitor (OSE-470 / 470L / 470M)

Compact and portable, no computer required, for observation results during electrophoresis detection after nucleic acid extraction

7. Full wavelength spectrophotometer (OSE-260-01 / 02)

Accurate and fast for UV spectrophotometric detection after nucleic acid extraction

Viral nucleic acid detection protocol

1. One-step reverse transcription-fluorescence quantification kit (probe method) (FP314)

Quickly identify viral RNA in one step

2. Automated pipetting station (AP 400/600)

For rapid automatic dispensing after PCR system preparation

3. Electric pipette (OSE-EP)

Fast and accurate for manual dispensing of PCR systems and various other pipetting operations

4. Vortex Mixer (OSE-VX-01 / 02)

For rapid mixing after PCR system configuration

5. Mini Centrifuge (OSE-MC8)

Multi-purpose machine, suitable for instant centrifugation of different types of EP tubes

6. Microplate Centrifuge (OSE-MP25)

For direct centrifugation of multiple eight-row tubes or 96/384 plates

Other supporting instruments

Clean bench

Brocade, Fuxia

Biological safety cabinet

Brocade, Fuxia

Autoclave

Brocade, Shenan, Xinhua Medical, Shanghai Sanshen

Air sterilizer

Brocade, Green Angel / Jixing, Aojie, Xinhua Medical

Low temperature refrigerator

PHC, Haier, Thermo

freezer

Haier, Boko

For customers who need to inquire or place an order please feel free to contact international@molbase.com