rhodamine B

CAS No. 81-88-9

Formula: C28H31ClN2O3
Basic Info

Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA.
Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably Mycobacterium.
Rhodamine B is tunable around 610nm when used as a laser dye. Its luminescence quantum yield is 0.65 in basic ethanol, 0.49 in ethanol, 1.0, and 0.68 in 94% ethanol. The fluorescence yield is temperature dependent.

Formula
C28H31ClN2O3
Molecular Weight
479.01
Exact Mass
478.202
LogP
2.565
PSA
56.69
Synonyms

C.I. Basic Violet 10

rhodamine hydrochloride

Rhodamine B

Pilot578

AKA213

rhodamine blue

AKA214

9-(2-carboxyphenyl)-3,6-bis(diethylamino)xanthylium chloride

c.i.749

D and C Red No. 19

redno213

Basic Violet 10

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Properties
Appearance & Physical State
red to violet powder
Density
1.31
Melting Point
210-211ºC (dec.)
Flash Point
12ºC
Water Solubility
SOLUBLE
Stability
Stable. Incompatible with strong oxidizing agents.
Storage Condition
Store at RT.
Safety Info
RTECS
BP3675000
Safety Statements
S26-S36/37/39
WGK Germany
2
Risk Statements
R22; R41; R68
HS Code
32041300
RIDADR
UN 1219 3/PG 2
Caution Statement
P210; P261; P305 + P351 + P338
Hazard Codes
Hazard Declaration
Spectrum
NMR Spectrum 1H NMR : 400 MHz in DMSO-d6
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IR Spectrum IR : nujol mull
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Mass Spectrum Mass spectrum (electron ionization)
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Synthesis Route
1.Synthesis Route
2.Synthesis Route
3.Synthesis Route
Toxicity
ACUTE TOXICITY DATA
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Type of Test
LDLo - Lowest published lethal dose
Exposure Route
Oral
Species Observed
Rodent - rat
Dose/Duration
500 mg/kg
Toxic Effects
Details of toxic effects not reported other than lethal dose value--
Reference
National Academy of Sciences, National Research Council, Chemical-Biological Coordination Center, Review. (Washington, DC) Volume(issue)/page/year: 5,8,1953
ACUTE TOXICITY DATA
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Type of Test
LD50 - Lethal dose, 50 percent kill
Exposure Route
Intraperitoneal
Species Observed
Rodent - rat
Dose/Duration
112 mg/kg
Toxic Effects
Details of toxic effects not reported other than lethal dose value--
Reference
Toxicological European Research. (S.P.E., 10, Ave. de Messine, 75008 Paris, France) V.1- 1978- Volume(issue)/page/year: 1,23,1978
ACUTE TOXICITY DATA
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Type of Test
LD50 - Lethal dose, 50 percent kill
Exposure Route
Intravenous
Species Observed
Rodent - rat
Dose/Duration
89 mg/kg
Toxic Effects
Details of toxic effects not reported other than lethal dose value--
Reference
Toxicology and Applied Pharmacology. (Academic Press, Inc., 1 E. First St., Duluth, MN 55802) V.1- 1959- Volume(issue)/page/year: 3,696,1961

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